Effects of the In ovo Administration of L-Ascorbic Acid on Tissue L-Ascorbic Acid Concentrations, Systemic Inflammation, and Tracheal Histomorphology of Ross 708 Broilers Subjected to Elevated Levels of Atmospheric Ammonia

The effects of in ovo injection L-ascorbic acid (L-AA) on tissue L-AA concentrations, systemic inflammation, plasma mineral and tracheal histomorphology Ross 708 broilers subjected to elevated atmospheric ammonia (NH3) levels after hatch were investigated. four treatments included non-injected (control), saline-injected or saline containing 12 25 mg L-AA. applied at 17 days incubation by injecting a 100 μL volume each pre-specified treatment into the amnion. At hatch, male chicks randomly allocated replicate battery cages belonging group. arranged randomized complete block design within common room. All birds exposed 50 ppm NH3 35 posthatch age (doa), concentration room was recorded every 20 s. 0, 7, 14, 21, 28 doa, one bird from cage arbitrarily selected euthanized for determinations liver eye concentrations doa; nitric oxide calcium trace 0 21 doa. Tracheal evaluations performed There no significant differences sampling timepoint. In either decreased attenuation incidence doa compared control groups. Furthermore, percentage mild inflammation scores lower response These results indicate that reduces NH3.